Device
Part:BBa_K672000:Design
Designed by: UTP-Panama Team Group: iGEM11_UTP-Panama (2011-09-17)
Nitrate sensing at low temperatures.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 394
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2298
Design Notes
We wanted to make sure that both parts worked in our specific conditions, and for that we did tests in the lab as shown in the experience.
Source
It comes from the combination of two other existing Biobricks:
Part:BBa_K381001 *Pyear + RBS + GFP + TT + TT
Part:BBa_K410000 *hyBb + ompA + AOX
References
Pyear promoter
- Lin, H.-Y., Bledsoe, P.J., and Stewart, V. 2007. Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen-responsive regulator Fnr in Escherichia coli K-12. J. Bacteriol. 189, 7539-7548
GFP
Aox
- Grant, N et al. “Two Cys or Not Two Cys? That is the Question; Alternative Oxidase in the Thermogenic Plant sacred Lotus.” Plant Physiology 150 (2009): 987-995. {PAPER}
- HybB Sequence:[[1]]
- OmpA Sequence:http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/e8283dat.Par.0001.File.tmp/e8283dat.pdf
- AoxA Sequence:http://www.ncbi.nlm.nih.gov/nuccore/226897260#sequence_226897260